Wednesday, January 5, 2011

"Teka-meneka markah full punya"

                             Bismillahirrahmanirrahim ...


           InsyaALLAh bertemu lg dalam rancangan "Teka-meneka markah full Punya "
      dan saya memohon copyright daripada "Section 3 Cool" untuk menggunakan
                                    gambar2 yg telah ditembak mereka ...
     * Tulisan ini ditujukan khas buat saya sendiri atau sesiapa yg nak baca saje2... 
         dan boleh rujuk http://pharmicrosec3.blogspot.com utk kegunaan pejabat
     
                                          Harap x marah ...

                      So, tajuk pertama kita adalah
               Spore bearer bacilli (Pengandung Spore)










Spore Bearer Bacilli

                      


 Stain - GRAM STAIN +ve

          ( setahu saya sume Spore bearer +Ve je kot)





               protoplasm - deeply coloured/violet (sahaja)                             xxxxxx*spore is not stained*xxxxx     




Spore Bearer Bacilli

  


                    MODIFIED ZIEHL-NEELSEN STAIN 
                                   (Modified TB stain)
                      - Organism ada 2 warna
                      - Merah di tgh2 / Terminal / subterminal    
                        Protoplasm (violet)
                      - Satu2nya slide yg ada 2 colour
                --> B.antrax = spore in center
                -> C.Tetani=Spore large at terminal end(drumstick)
                --> C.perfringens = Oval not bulging, subterminal
              --> C.Botulinum = Oval , slightly bulging , subterminal


Spore bearer bacilli - NEGROSIN FUCHSIN STAIN

protoplasm - unstained
DARK BACKGROUND (Negro)



ANAEROBIC JAR





COOKED MEAT MEDIUM (ROBERTSON'S MEDIUM)

Reducing substances arise from sterilized muscle tissue which are :
  • blood pigments of the meat
  • unsaturated fatty acids
  • Growth of Bacteria :
  • - C.Tetani = Blackening of Medium (not stong proteolytic)
  • - C.perfringens = Reddened with sour smell 
  • (No proteolytic but saccharolytic) 
  • -C.Botulinum = Blackens cooked meat medium 
  • (proteolytic-liquify gelatin)



                                    DEEP NUTRIENT AGAR
                     - Broth + 2% agar (Stearilized by autoclaving at 120'c 20min)






Gram +ve diplococci : PNEUMOCOCCI
camne nak identify : SUka berdua2 berjauhan dari org lain
(bact. menjauhkan diri mereka dari masyarakat sekeliling)

*Kalau xnampak berjauhan pun jawab je Pneumococci kot
(as well as warna ungu bulat2)




Acid fast bacilli : MYCOBACTERIUM TUBERCULOSIS

stain - Ziehl Neelsen stain
bacilli - pink
pus - blue
*Smear ke culture ?
- Smear la  , sebab ada pus cell scattered in field
-Pink rod bact. present







Gram -ve bacilli
-Bacilli = Rod-shaped
-Gram -ve = Merah

contoh bacteria : Enterobacteriaceae
-Salmonella
-Shigella
-E.coli
-Klebsiella
-Protius






 NEISSERIA smear

Stain - Methylene blue
Smear : Pus Cell
Bacteria = Pun warna biru juga










Gram -ve diplococci : NEISSERIA "smear"

stain - Gram stain
DiploCOCCI = Bulat2 + ada pus cell
(Dua2 warna merah)





Gram -ve diplococci : NEISSERIA culture
Culture - Sebab xde Pus cell

Soalan .
Niesseria boleh buat sakit apa ?
-N.meingitidis - Meningitis
-N.Gonorrhea - Gonorrhea


Test tubes :



1. Lowenstein Jensen Medium (LJ)
Type : Selective Media (inhibit undesired organism to grow)
Content : Beaten Eggs , Mineral salts , 
Malachite Green (Kuih melaka warna hijau)

(Hmm..nyam nyam kalau ada org nak kasi mesti sedap)
-Tambah : Glycerol (Human type)
                     Pyruvate (Bovine type)





Egg saline
Type : Solid Media (Enriched MEdia)
Uses : For culture of TB

Plates :




1. Blood agar
Uses : Growing delicate or fastidious organisms 
                : Differentiate Organism for haemolytic action




2. Chocolate agar
Uses : For culture of Haemophylus n Niesseria


FUNGUS pula 



Candida
Bentuk - Bulat2 (Yeast-Like)
=sepatutnya ada pseudomycelium tp xnampak la pulak

apa Disease yg di Boleh buat ?
- Oral candidosis
-Oesophageal candidosis
-Enteric candidosis
Perianal Candidosis
Candida intertigo
Onychia and Paronychia
Vaginal and vulvo-vaginal Candidosis
Bronchial and pulmonary Candidosis
Renal Candidosis
Candida Endocarditis
Candida Septicaemia
Candida Meningitis
Chronic mucocutaneous Candidosis

*Senangkata hantam saja apa2 penyakit , cuma tambah candida
Ok kot..




 

Aspergillus niger

Niger = Hitam
- Long condidiophore
-Septate mycelium
-Rounded Vesicles
- Biseriate phialides

-Conidia
(5% of the disease)




Aspergillus fumigatus
Fumi - Olive-Green (Buah zaitun)
- Long condidiophore
- Club-shaped Vesicles
-Uniseriate phialides

Disease can be produced By Asp. spp
- Aspergilloma
-ABPA (Allergic bronchopulmonary aspergillosis)
- Bronchial Asthma
-Extrinsic alveolitis
Invasive Pulmonary aspergillosis
-Peural Aspergillosis

* Mainly asparagus ni buat penyakit dkt lung 





Macroconidia of Microsporum canis

- A dermatophytes (under group microsporium)
- Macroconidia = Multiseptate , variable in form , big sized , irregular surface
- Attack mainly = Hair n Skin
* Can cause Gray-Patches


Photos




Candida culture in test tube

- Culture = Saboraud Dextrose Agar 

Content :- Dextrose
             -Peptone
         -Agar
        -H2O

+ Chloramphenicol
- Cycloheximide xyah =sbb Candida rapidly growing




Thrush stomatitis

Causatic agent : Candida Albicans
Usually Seen : on diabetic patient + Debilitated aged
                  - Intensive antibiotic n Corticosteroid



Endothrix (black dot)

Causatic agent :Tinea Capitis
Species : 
- T.Tosurans (insurans)
- T.violaceum (Biol la-pening kepala)




Ectothrix (gray patch)

- Gambar orang tgh masukkan jari kedalam hidung (errk..main2 je)

Causatic Agent : Tinea Capitis juga...
Species :
- M.Canis (Mohd Hanis)
- M.audounii (mama adodoi ...)



Ectothrix (cotton blue)
-sama macam atas

Stain = Lactophenol-cotton blue



Tinea capitis (favus)
-T.Capitis @ T. Favosa
--> Most severe disfiguring form

species : T.Schoenleinii (nk pg school la ni ..)



Tinea corporis

Affected site : Body (glabrous skin)



Onychomycosis

Agent : T.Unguium
Species :
- T. Rubrum (brum..brumm..)
           - T.Mentagrophyte (mintak goreng ketupat)



Plates





Candida
- Bulat2 yeast-like
- Mentioned above




Aspergillus niger
Niger - Hitam
- Explained above




Aspergillus fumigatus
- Sehijau warna zaitun (olive-green)



Tambahan Satu lg...



Ok ..Gudluck semua

"Mumtaz for Ummah InsyaALLAH"

*Semua yg ditulis tiada berkenaan sama ada yg hidup atau yg sudah meninggal dunia...

*Credit to Section 3 


Your Brother in Islam
-Islahmujahidin-

7 comments:

sepi angin ke kota iskandariah said...

insya Allah sem depan, section 4 akan buat blog baru, so bole la dijemput untuk jadi penulis panel...
btw, syukran....

Anonymous said...

syukran awi2

teman said...

Terima kasih abang Syahmi..
Semoga berjaya dengan cemerlang..

Anonymous said...

tq syahmi...
exam besok ak nk dok sblh ko boleh??
gudluck!!!

Anonymous said...

hi abang cute comel...hoohoh
tq banyak2... lombat..lombat.. lombat apek lombat
haaha

.p0 said...

ty weyh

Nor Syaqinah Razali said...

syukran awi..
berguna sangat
moga dipermudahkan menjawab exam.